Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer’s Disease and Mild Cognitive Impairment Patients

Figure 3

MnSOD level and its nitrated isoform in PBMCs derived from AD, MCI patients and healthy controls. Protein extracts derived from PBMCs of AD, MCI, and control were processed for Western blot analysis and immunoprecipitation assay (ip). (a) A representative immunoblot of protein extracts derived from 2 controls, 2 AD, and 2 MCI carried out with anti-MnSOD antibody. Tubulin was used to normalize the samples. (b) The 3NT-enriched fractions and 3NT-free washed fractions derived from ip experiments of the same 2 controls, 2 AD, and 2 MCI were loaded onto 12% SDS-PAGE gels and immunoblotted with anti-MnSOD antibody. (c) Quantitative analysis of MnSOD levels of 6 AD, 6 MCI, and 6 control samples expressed as MnSOD expression over α-tubulin levels. (d) Quantitative analysis of MnSOD nitrated isoform over 3NT-free isoform performed on 6 AD, 6 MCI, and 6 control samples. Data were expressed as mean ± SEM. The statistical significance was represented by the asterisk versus the corresponding control group.

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