Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells

Figure 2

Oregano essential oil (OEO) inhibits hydrogen peroxide- (H₂O₂-) induced reactive oxygen species (ROS) and malondialdehyde (MDA) production in IPEC-J2 cells. (a) Cells were pretreated with OEO (2.5–10 μg/mL) for 24 h and then incubated with 0.8 mM H₂O₂ for 24 h. Intracellular ROS levels were indicated by DCF fluorescence and detected by fluorescence microscopy (200x magnification). (b) Cells were pretreated with OEO (2.5–10 μg/mL) for 24 h and then incubated with 0.8 mM H₂O₂ for 24 h. The fluorescence intensity of DCF-stained cells was quantified by flow cytometer. (c) Cells were pretreated with OEO (2.5–10 μg/mL) for 24 h and then incubated with 0.8 mM H₂O₂ for 24 h. The levels of ROS in culture supernatant were measured by chemiluminescence. Chemiluminometric counts were obtained at 0.05 sec intervals for 5 sec, and the results were expressed as areas under the curve of relative light units (RLU) for 5 sec. (d) Cells were pretreated with OEO (2.5–10 μg/mL) for 24 h and then incubated with 0.8 mM H₂O₂ for 24 h. The intracellular MDA levels were measured. Values represent means ± SEM, . Means without a common letter differ, .

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