Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells

Figure 6

Nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA diminishes the protective effects of IPEC-J2 cells. IPEC-J2 cells were treated with Nrf2 siRNA or control siRNA and incubated for 24 h. Cells were incubated with oregano essential oil (OEO, 10 μg/mL for 24 h) and then incubated with H₂O₂ (0.8 mM) for 24 h. (a) Cell viability was determined by MTT assay. (b) Intracellular reactive oxygen species (ROS) levels were measured by DCF fluorescence microscopy. Values represent means ± SEM, , significant at , .

(a)

(b)