Alternatively Spliced Methionine Synthase in SH-SY5Y Neuroblastoma Cells: Cobalamin and GSH Dependence and Inhibitory Effects of Neurotoxic Metals and Thimerosal

Figure 3

MS activity in SH-SY5Y cells is GSH-dependent and requires MeCbl. (a) Methylfolate-dependent MS activity in the presence of MeCbl or OHCbl ± SAM. versus MeCbl. versus OHCbl/SAM group. (b) Methylfolate-dependent MS activity following inhibition of GSH synthesis with BSO (1 mM; 24 hrs). versus no BSO treatment. (c) MS activity in the presence of GSH (1 mM) or DTT (1 mM) following BSO treatment. versus BSO-only. (d) Methylfolate-dependent MS activity in the presence of GSCbl ± SAM ± BSO treatment. versus untreated group.

(a)

(b)

(c)

(d)