Research Article
Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
Figure 6
The protective effects of GSPB2 on oxidative stress-induced cytotoxicity in granulosa cells. Granulosa cells were pretreated with various concentrations of GSPB2 for 24 h and then exposed to H2O2 (150 μM) for 6 h. (a) Effects of GSPB2 on the viability of granulosa cells treated with H2O2. (b) Effects of GSPB2 on the intracellular ROS levels. (c) Quantification of intracellular ROS levels. (d) Effects of GSPB2 on the apoptosis of granulosa cells. (e) Quantification of the apoptosis rates. (f) Effects of GSPB2 on the mRNA levels of apoptosis-related genes. Scale bars are 100 μm. Data was shown as mean ± SEM, . , H2O2 alone group versus H2O2-free group (control); versus H2O2 alone group.
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