Research Article
Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
Table 3
Primer sequences for RT-PCR.
| | Gene | Accession number | Primer sequences (5′-3′) | Product size (bp) | Annealing temperature (°C) |
| | GAPDH | NM_008084.2 | F: ATGGTGAAGGTCGGTGTGAACG
R: CTCGCTCCTGGAAGATGGTGATG | 452 | 56 |
| | Bax | NM_007527.3 | F: CCAGGATGCGTCCACCAAGA
R: GGTGAGGACTCCAGCCACAA | 394 | 57 |
| | Bcl-2 | NM_009741.3 | F: GTGGATGACTGAGTACCTGAACC
R: AGCCAGGAGAAATCAAACAGAG | 120 | 60 |
| | Bim | NM_009754.3 | F: TATGGAGAAGGCATTGAC
R: TGTGGTGATGAACAGAGG | 207 | 56 |
| | Caspase-3 | NM_009810.2 | F: ACAGCACCTGGTTACTATTC
R: CAGTTCTTTCGTGAGCAT | 255 | 54 |
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