Hyperbilirubinemia Protects against Aging-Associated Inflammation and Metabolic Deterioration

Figure 2

Bilirubin has no negative effect on mitochondria or viability of cultured cells. (a) Whole cell respiration of T-Rex-293 cells treated with bilirubin for 24 hours, measured with respirometry; (b) mitochondrial membrane potential in T-Rex-293 cells treated with bilirubin for 24 hours, measured with tetramethylrhodamine dye. Uncoupling of mitochondria with 10 μM FCCP was performed as a positive control; (c) relative proliferation of T-Rex-293 cells treated with bilirubin for 72 hours, measured as a total cell protein; (d) relative viability of T-Rex-293 cells treated with bilirubin for 24 hours, measured with WST-1 assay. Data in panels (c and d) expressed as % of controls; in each group; significance in panel (a and b) measured with -test and overall significance in panel (c and d) measured with ANOVA.

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