Skin Aging-Dependent Activation of the PI3K Signaling Pathway via Downregulation of PTEN Increases Intracellular ROS in Human Dermal Fibroblasts

<div>Replicative senescence-dependent increase in intracellular PIP<sub>3</sub> levels through PTEN downregulation. (a) Expression levels of PI3K and PTEN, in various PDs of HDFs. Various PDs of HDFs were analyzed by western blotting for PTEN, PI3K p85 subunit, and <i>β</i>-actin. (b) PIP<sub>3 </sub>immunofluorescence staining in various PDs of HDFs. Various PDs of HDFs were fixed with 4% paraformaldehyde, incubated with anti-PIP<sub>3 </sub>antibody and stained with TRITC-labeled anti-mouse IgM antibody. Images were acquired using an Olympus FluoView™ laser scanning confocal microscope.</div>

Oxidative Medicine and Cellular Longevity

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Figure 3

Figure 3: Skin Aging-Dependent Activation of the PI3K Signaling Pathway via Downregulation of PTEN Increases Intracellular ROS in Human Dermal Fibroblasts 

Figure 3 | Skin Aging-Dependent Activation of the PI3K Signaling Pathway via Downregulation of PTEN Increases Intracellular ROS in Human Dermal Fibroblasts 