Oxidative Medicine and Cellular Longevity / 2016 / Article / Fig 3

Research Article

The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction

Figure 3

H2S donor NaHS protects cardiomyocytes from hypoxia-induced injury. (a) Cell viability was detected with LDH and CCK8 kits. Hypoxia exposure decreased cell viability, as evidenced by high LDH activity and low CCK8 values; 30 min of NaHS pretreatment alleviated the above injury, as demonstrated by the significant drop in LDH activity and increase in CCK8 values; (b) H2S level in the culture medium decreased after exposure to hypoxia, and 25–100 μM NaHS pretreatment in a concentration-dependent manner caused elevation of H2S levels; (c) 6 h hypoxia exposure did not change CSE or 3-MST expressions but significantly increased CBS expression; (d) NaHS pretreatment did not change the expression of either CSE or 3-MST but increased CBS expression at 100 μM. (e) The activity of CSE/CBS declined after exposure to hypoxia, whereas 50 μM NaHS treatment caused a significant increase in their activity; (f) Hypoxia with or without NaHS treatment did not change the activity of 3-MST. Numbers inside bars denote the number of animals per group. Values are mean ± SE. and .