Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 1

Influence of the TPEN chelator on cell viability and intracellular zinc alterations. (a) Concentration-dependent studies for MTT assay on neuroblastoma. SH-SY5Y cells were treated with varying concentrations of TPEN, while untreated cells were used as control. (b) Concentration-dependent studies for Trypan Blue assay on neuroblastoma. SH-SY5Y cells were treated with varying concentrations of TPEN, while untreated cells were used as control. (c) The concentration of zinc was determined by flame atomic absorption spectroscopy. Data represent the mean values ± standard deviation (), and significant differences between treated and untreated cells were and , respectively.

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