Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 2

Apoptosis analysis. (a) Dot plot of SH-SY5Y after exposure to 100 nM staurosporine for 4 hours (positive control). Dot plot graphs from left to right show cells treated with (1) 100 nM staurosporine labeled with Annexin V-FITC, (2) 100 nM staurosporine labeled with PI, and (3) 100 nM staurosporine labeled with Annexin V-FITC and PI. (b) Dot plot of SH-SY5Y after exposure to 5 or 25 μM TPEN for 12, 24, and 48 hours and flow cytometry analysis with Annexin V-FITC versus PI. The divisions of the plots distinguish necrotic cells (Annexin V⁻/PI⁺, left upper quadrant) from early apoptotic cells (Annexin V⁺/PI⁻, right lower quadrant) and late apoptotic cells (Annexin V⁺/PI⁺, right upper quadrant). The plots in the figure are representative of five independent experiments. Data represent the mean values ± standard deviations () and significant differences between untreated cells and cells treated with TPEN 25 μM.

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