Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 3

ROS Analysis. (a) Intracellular fluorescence of 2′,7′-dichlorodihydrofluorescein (DCF) in SH-SY5Y cells that were treated or not with 5 or 25 μM TPEN for 12, 24, and 48 hours measured by FACS (a1) flow cytometric data compiled on a single graphic showing no change in DCF fluorescence in different incubation time and concentration of TPEN (a2). Viable cells were labeled with PI to exclude dead cells and only viable cells were analyzed. (b) Dihydroethidium (DHE) in SH-SY5Y cells treated with 5 or 25 μM TPEN for 12 (b1), 24 (b2), and 48 hours (b3). Flow cytometric data compiled on a single graphic showing no change in DHE fluorescence in different treatment time and concentration of TPEN (b4). Data represent the mean values ± standard deviations (). Significant differences between positive control (DMNQ) and treated cell were . There were no significant differences between untreated and treated cells.

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