Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 6

Influence of the TPEN chelator in the expression levels of apoptotic proteins. SH-SY5Y cells were treated with 25 μM TPEN. At each time point, 100 μg of total proteins from the total cell lysates was loaded onto each lane for the detection of p-p38 (a), Bax (b), Bcl-2 (c), JNK (d), and p-JNK (e). β-actin and α-tubulin were used as a loading control. The Western blot images represent three independent experiments, and significant differences between untreated and treated cells were and , respectively.

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