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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 6731093, 14 pages
Research Article

Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

1Division of Nephrology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 137701, Republic of Korea
2Department of Internal Medicine, Incheon St. Mary’s Hospital, Incheon, Republic of Korea
3Department of Internal Medicine, Seoul St. Mary’s Hospital, Seoul, Republic of Korea
4Department of Internal Medicine, Yeouido St. Mary’s Hospital, Seoul, Republic of Korea

Received 16 December 2015; Revised 8 March 2016; Accepted 31 March 2016

Academic Editor: Andreas Daiber

Copyright © 2016 Hye Eun Yoon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice. Methods. Histologic changes and expressions of transforming growth factor-β (TGF-β), collagen IV, fibronectin, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG), 3-nitrotyrosine, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice. Results. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-β, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine1177-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change. Conclusions. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice.