Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer
LXA4 reverses CoCl2-induced cell invasion through ROS/ERK/MMP pathway. (a) Effect of LXA4 on CoCl2-induced cell invasion. Panc-1 cells were treated with vehicle (methanol), LXA4, CoCl2 (0.15 mM), or CoCl2 + LXA4. Cell invasion assay was performed when cells had been transferred into transwell chamber for 48 hours. (b) The quantified data of (a). (c) Western blot analysis of cells treated as above. (d) Intracellular ROS determined in cells treated in (a). Cells incubated with DCF-DA for 20 min were washed with PBS three times and then lysed by RIPA lysis buffer and tested by fluorimetry at 510 nm. The absorbance was normalized by total protein. (e) Expression of activated p-ERK and total ERK detected by western blot. versus corresponding control.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.