The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress

Table 2

Experimental design.

Sublethal oxidative stress, induction of premature senescence
Experimental setup	Stress phase (2 hours)	Recovery phase (up to 15 days)	Tested EGCG effect
(1) Antioxidant	50 μM H2O2 + 10 μM EGCG	—	ROS neutralization
(2) Recovery	50 μM H2O2	+10 μM EGCG	Interaction with poststress signaling; (e.g., changes in gene expression)
(3) Combined	50 μM H2O2 + 5 μM EGCG	+5 μM EGCG	ROS neutralization; Interaction with poststress signaling
Lethal oxidative stress, cell death induction
Experimental setup	Stress phase (24 hours)		Tested EGCG effect
(1) Survival	100 and 200 μM H2O2 + 10 μM EGCG		Prosurvival; Antioxidant