Punicalagin Induces Serum Low-Density Lipoprotein Influx to Macrophages

Figure 3

LDL or oxLDL influx into macrophages. (a) The ability of glabridin or punicalagin (2 μM) to induce LDL influx to macrophages. (b) FACS histogram of macrophages incubated with LDL-FITC upon addition of 2 μM punicalagin (purple), glabridin (blue), quercetin (green), or catechin (light-blue). Note that only punicalagin affects LDL influx as displayed by the curve shift compared to the control curves (red, brown, and black) representing macrophages incubated with LDL-FITC (positive control), LDL (negative control), and FITC-conjugated BSA (negative control), respectively. (c) FACS histogram of macrophages incubated with LDL-FITC (red) upon addition of 2 μM punicalagin (purple) or oxLDL-FITC (brown) upon addition of 2 μM punicalagin (green). LDL cellular fluorescence was measured in mean fluorescence intensity (MFI) or percent fluorescent cells.

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