HDAC6 Regulates the Chaperone-Mediated Autophagy to Prevent Oxidative Damage in Injured Neurons after Experimental Spinal Cord Injury

Figure 3

Deficiency of HDAC6 accelerates intracellular ROS generation in response to HI. The PC12 and PC12/siHDAC6 cells were treated with HI for 24 h. Ctrl express vehicle-treated group. Changes in the intracellular DCF fluorescence value and the percentage of cell with fluorescence can be detected by flow cytometry. The -axis indicates the number of cells with fluorescence; the -axis expresses fluorescence intensity. Red indicates the level of intracellular ROS. Green indicates the figure of %Gated. Result of one representative experiment was shown. The densitometric quantification is represented as mean ± SEM (), versus control group; , indicate the significance among the groups indicated in the bar chart.