SIRT1/3 Activation by Resveratrol Attenuates Acute Kidney Injury in a Septic Rat Model

Figure 6

Mitochondrial morphology and function of renal tubular epithelial cells after CLP. (a) Representative transmission electron microscope images of the mitochondria. Healthy mitochondria (control group) have intact mitochondrial membranes and cristae. Mitochondria are swollen, with poorly defined cristae and more mitochondrial vacuolization in the vehicle group, whereas these changes are prevented partially in RSV group. (b) Representative flow cytometry scatter plots of the JC-1 probe. Cells were stained with the JC-1 probe; regions of reduced polarization are indicated by the JC-1 monomers. Regions of high polarization of mitochondrial membranes are indicated by the formation of J-aggregates. (c) was monitored using JC-1 by flow cytometry and mitochondrial depolarization was quantified as JC-1 aggregates/monomers. (d) ATP level was determined using a System Bioluminescence Detection kit. (e) Flow cytometric quantification of calcein-AM fluorescence. Intensity of calcein-AM fluorescence reflects the extent of opening of the mPTP. Values are presented as the mean ± SEM. versus the control group; and versus the vehicle group; versus the RSV group. . RSV, resveratrol; ATP, adenosine triphosphate; , mitochondrial membrane potential.

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