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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 7323627, 11 pages
http://dx.doi.org/10.1155/2016/7323627
Research Article

Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

1Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil
2Laboratory of Inflammation and Behaviour Pharmacology, Pharmacy Faculty, Institute of Health Science, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil
3Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil
4Department of Oral Pathology, School of Dentistry, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil

Received 8 March 2016; Revised 1 July 2016; Accepted 5 July 2016

Academic Editor: Francisco J. Romero

Copyright © 2016 Nathalia Carolina Fernandes Fagundes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigates morphological and biochemistry effects of binge ethanol consumption in parotid (PG) and submandibular (SG) salivary glands of rats from adolescence to adulthood. Female Wistar rats () received ethanol at 3 g/kg/day (20% w/v) for 3 consecutive days/week from the 35th until the 62nd day of life. Animals were treated in two periods: 1 week (G1) and 4 weeks (G2), with a control (treated with distilled water) and an ethanol group to each period. In morphological analysis, morphometric and immunohistochemistry evaluation for smooth muscle actin (αSMA), cytokeratin-18 (CK-18), and vimentin (VIM) were made. Biochemical changes were analyzed by concentration of nitrites and levels of malondialdehyde (MDA). The difference between groups in each analysis was evaluated by Mann-Whitney U test or Student’s -test (). PG showed, at one week of ethanol exposure, lower CK-18 and α-SMA expression, as well as MDA levels. After four weeks, lower CK-18 and higher MDA levels were observed in PG exposed to ethanol, in comparison to control group. SG showed lower α-SMA expression after 1 and 4 weeks of ethanol exposure as well as higher MDA levels after 1 week. Ethanol binge consumption during adolescence promotes tissue and biochemical changes with only one-week binge in acinar and myoepithelial PG cells.