Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

Figure 4

Effect of APO (2 mM) and DPI (100 nM) on the Stat3, Akt, and Erk phosphorylation in serum starved mES cells (SF) treated by drugs for 20 minutes followed by LIF (5 ng/mL) or FBS (15%) stimulation for 20 minutes. Total level of β-actin was used as a loading control. A typical representative western blot is shown.