Oxidative Medicine and Cellular Longevity / 2016 / Article / Fig 5

Research Article

CD38 Deficiency Protects the Heart from Ischemia/Reperfusion Injury through Activating SIRT1/FOXOs-Mediated Antioxidative Stress Pathway

Figure 5

CD38 deficiency promotes expressions of NOX4 and decreases overloading of intracellular Ca2+. (a) Representative images of the gating plot and peaking plot of intracellular Ca2+ stained with fluo-3AM. The fluorescent dye of fluo-3AM was loaded into myocardial cells and the fluorescent intensity was measured by flow cytometry assay. Although there was a robust elevation of Ca2+ in CD38 knockdown and control H9c2 cells after I/R, the concentration of intracellular Ca2+ in the CD38 knockdown cells was much lower than that of control cells. (b) Quantification of the mean fluo-3 fluorescence intensity in the different groups of cells. (c–e) Expressions of NOX4 in myocardial cells. The relative mRNA and protein expressions of NOX4 in normal and CD38 knockdown H9c2 cells were determined by qPCR (c) and western blot with NOX4 antibody (d, e), respectively, and GAPDH expression was also detected as a loading control. Data were presented as mean ± SD, , and and .
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