Antioxidant Activity and Cytotoxicity Effect of Cocoa Beans Subjected to Different Processing Conditions in Human Lung Carcinoma Cells

Table 2

Effect of extracts of cocoa nibs roasted slates and unroasted well fermented cocoa (5–10 mg/mL) on programmed cell death in human lung cancer cell line after 48 hours.

Stages of cell death	CT	RS (mg/mL)		UWF (mg/mL)
		5	10	5	10
Viable cells; (Annexin V−/PI−)	95.80 ± 0.57	89.10 ± 2.26	86.73 ± 1.96	91.37 ± 1.33	72.03 ± 4.67
Early apoptosis; (Annexin V+/PI−)	2.16 ± 1.03	1.80 ± 0.83	8.22 ± 2.48	3.87 ± 1.22	4.85 ± 2.05
Late apoptosis; (Annexin V+/PI+)	0.90 ± 0.25	2.56 ± 1.30	4.92 ± 0.95	2.23 ± 0.67	8.13 ± 0.06
Nonapoptotic cells; (Annexin V−/PI+)	1.12 ± 0.69	6.54 ± 0.13	0.14 ± 0.69	2.52 ± 0.68	14.93 ± 0.42

Results are expressed as percentage of total cells. The experiment is expressed as mean ± standard deviation; significant differences between untreated cells (CT) and cells treated with lycopene (5–10 μM) were compared by one-way ANOVA with the posttest of Tukey (; ).