Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROS/p53/p21^Cip1/Waf1 Pathway

Figure 2

The detection of SA-β-gal activity and ROS level in BMSCs. (a) Representative image of SA-β-gal staining in the P3 BMSCs in the absence (control, Ctl) or presence of CH (5, 10, and 15 μg/mL). (b) Percentage of SA-β-gal positive senescent cells in different groups shown in (a). for each group, versus Ctl group. (c) MTT results of BMSCs with a variety of concentration of H₂O₂. for each group, versus Ctl group. (d) Percentage of SA-β-gal positive cells in the P1 BMSCs treated with H₂O₂ or H₂O₂ + different concentrations of CH. for each group, versus Ctl group, , versus H₂O₂ group. (e) ROS level in BMSCs in different groups. for each group, versus P1 group, , versus P3 group. (f) ROS level in the BMSCs treated with H₂O₂ or H₂O₂ + different concentrations of CH. for each group, versus Ctl group, versus H₂O₂ group.

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