Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1

Figure 1

Ox-LDL suppressed SIRT1 expression and impaired autophagic flux in HUVECs. Western blotting analysis showed that Ox-LDL decreased SIRT1, increased LC3-II, and increased p62 in a dose-dependent manner after Ox-LDL treatment of HUVECs for 12 h (a, b). Actin served as loading controls. (c) The relative mRNA level of p62 assessed by reverse transcription PCR. (d) Cell viability was determined by a CCK-8 assay; cell survival was given as percentage of control. Western blotting analysis showed that Ox-LDL (60 μg/mL) decreased SIRT1, increased LC3-II, and increased p62 in a time-dependent manner in HUVECs (e, f). (g) HUVECs were treated with 60 μg/mL Ox-LDL in the presence of Baf A1 (50 nM), and LC3-II level was evaluated by western blot analysis. Data are expressed as mean ± SEM; = (3–6); , versus controls of SIRT1; , , and versus controls of LC3II; , , and versus controls of p62; NS: not significant.

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