Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1

Figure 2

Resveratrol increased the expression of SIRT1 and enhanced autophagic flux in HUVECs. (a) Cell viability was determined by CCK-8 assay after treatment of HUVECs with different concentrations of resveratrol for 12 h. Western blotting analysis showed that resveratrol increased the expression of SIRT1 and LC3-II and decreased that of p62 in a dose-dependent manner (b, c). Resveratrol (50 μM) increased the expression of SIRT1 and LC3-II and decreased that of p62 in a time-dependent manner (d, e). Actin served as loading controls. Data are expressed as mean ± SEM; = (3–7); , , and versus controls of SIRT1; , , and versus controls of LC3II; , versus controls of p62; NS: not significant.

(a)

(b)

(c)

(d)

(e)