Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway

Figure 3

Effect of SFN administration on Nrf2 and Nrf2-regulated antioxidant enzyme expression and production of ROS during cerulein-induced AP. (a) The mRNA levels of Nrf2 after SFN treatment in cerulein-stimulated pancreatic acinar cells were measured by quantitative real-time PCR. (b) Expression of protein of nuclear Nrf2 by SFN in cerulein-treated pancreatic acinar cells was measured by western blot. (c) The mRNA expression of NQO1, HO-1, SOD1, and GPx1 after SFN treatment in cerulein-induced pancreatic acinar cells was measured by quantitative real-time PCR. (d) Lastly, the effect of SFN on the production of ROS in cerulein-treated pancreatic acinar cells was detected by fluorescence absorbance. Values are expressed as mean ± SEM. and when cerulein-induced group was compared with control group. , , and when SFN-treated group was compared to cerulein-induced group.

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