Research Article

Hericium erinaceus Inhibits TNF-α-Induced Angiogenesis and ROS Generation through Suppression of MMP-9/NF-κB Signaling and Activation of Nrf2-Mediated Antioxidant Genes in Human EA.hy926 Endothelial Cells

Figure 4

HE inhibits TNF-α-induced ICAM-1 expression in EA.hy926 cells. (a) Cells were harvested after pretreatment with the indicated concentration of HE (10–200 μg/mL) for 2 h in the absence or presence of TNF-α (10 ng/mL) for 4 h. (b) Cells were pretreated with HE (100 μg/mL) for 2–8 h in the presence or absence of TNF-α (10 ng/mL for 4 h). An equal amount (50 μg) of total lysate from each sample was resolved by 12% SDS-PAGE with β-actin as a control. Relative changes in protein bands were measured using densitometric analysis. Results are presented as mean ± SD of three independent assays; indicates significant difference compared to control, and indicates significant difference compared to TNF-α alone treatment.