Hepatitis C Virus NS5A Protein Triggers Oxidative Stress by Inducing NADPH Oxidases 1 and 4 and Cytochrome P450 2E1

Figure 3

HCV NS5A protein induces expression of cytochrome P450 2E1 which contributes to ROS production. Huh7 cells were transfected with NS5A-expressing constructs and subjected to quantification of CYP 2E1 levels by real-time RT-PCR (a) or Western blotting (b) 40 hrs after transfection. (c) 18 hrs after transfection NS5A expressing Huh7 cells were treated with 100 μM 4-methylpyrazole (4MP) for additional 12 hrs prior to quantification of ROS levels in DHE assay. Results are mean ± SD from six replicates; versus DMSO-treated cells transfected with pVax1; (ANOVA with Tukey-Kramer post hoc analysis).

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