Hepatitis C Virus NS5A Protein Triggers Oxidative Stress by Inducing NADPH Oxidases 1 and 4 and Cytochrome P450 2E1

Figure 4

ER oxidoreductins 1α and 1β and calcium signaling have no significant role in induction of oxidative stress by NS5A protein. (a) Huh7 cells were transfected with NS5A-expressing plasmids, and levels of Ero1α and 1β transcripts were analyzed 40 hrs after transfection by real-time RT-PCR. (b, c) 18 hrs after transfection NS5A expressing Huh7 cells were treated with calcium chelator BAPTA-AM or inhibitor or mitochondria Ca²⁺ uniporter Ru360, and ROS levels were analyzed 28 hrs after transfection in DHE (b) or H₂DCFDA (c) assays as described in Section 2. Results are mean ± SD from six replicates; versus DMSO-treated cells transfected with pVax1; between the indicated groups (ANOVA with Tukey-Kramer post hoc analysis).

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