Assessment of Mitochondrial Dysfunction and Monoamine Oxidase Contribution to Oxidative Stress in Human Diabetic Hearts

Figure 3

Atrial H₂O₂ emission detected with DCF fluorescence production. Fluorescent green positive staining was measured by confocal microscopy in the dichlorofluorescein- (DCF-) treated images. Levels of H₂O₂ detected with DCF fluorescence were similar in the studied groups (/group). Spectral unmixing was done using Lambda scan acquisition mode, range 490–600 nm, step 10 nm. The resulting composite image shows the real DCF component (red channel) on the autofluorescent background (green channel).