Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation

Figure 2

OGDR affects Drp1 expression through ubiquitin proteasome system. (a) mRNA level of Drp1 in mouse N2a cells upon OGDR insult was assessed by quantitative real-time PCR. Actin mRNA was used as an internal control. (b) Mouse N2a cells were incubated in the absence or presence of MG132, a reversible proteasome inhibitor. Cell lysates were analyzed by western blot with antibodies against mitochondrial fission-specific Drp1. Actin was used as a loading control, and all blots are representative of three independent experiments. (c) N2a cells were transfected with parkin siRNA or control siRNA (Ctrl siRNA), and western blot was performed to examine the expression of parkin and Drp1 protein. (d) Drp1 protein level was quantified according to the results of three independent blots. Data represent the mean ± SEM. Asterisks indicate a statistically significant difference compared with the control. .

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