Impact of Antioxidants on Cardiolipin Oxidation in Liposomes: Why Mitochondrial Cardiolipin Serves as an Apoptotic Signal?

Figure 9

Prevention of MeO-AMVN-induced oxidation of CL liposomes by antioxidants added externally within POPG liposomes. Peroxidation was started by addition of 50 μM MeO-AMVN to 100 μM CL liposomes at 40°C in buffer A. Antioxidant-containing POPG liposomes were added 30 minutes after the start of the experiment, as indicated by arrows. (a) Comparison of SkQ1H₂ with natural hydrophobic antioxidants. In each case the final concentrations were 1 μM of antioxidants and 40 μM of POPG. (b) Radical quenching efficiency of ubiquinol-10 as function of its concentration and the extent of dilution. The amount of added POPG stock suspension was 1% (30 μL) or 5% (150 μL) of the total system volume, which accounts for 40 or 200 μM POPG, respectively.

(a)

(b)