Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1/p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats

Figure 4

Effects of NACA on p38 MAPK phosphorylation in kidney. IHC staining of phospho-p38 MAPK in CON (a), CIN (b), NAC+CIN (c), NACA1+CIN (d), and NACA2+CIN (e) rats, respectively (figures are representative of 5 to 8 rats in each group). Note positive stained area (yellow) of IHC staining (arrow). (f) Semiquantitative analysis of phosphor-p38 MAPK expression in kidneys with IHC. (g) Phospho-p38 MAPK and total-p38 MAPK expressions by western blotting ( each). (h) Relative densitometry analysis of the ratio of phospho-p38 MAPK to total-p38 MAPK. Data are shown as means ± SD ( each). versus CON, versus CON, versus CIN, and ^& versus CIN+NAC.

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