Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1/p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats

Figure 5

NACA blocked CM-induced p38 MAPK activation by inhibiting ASK1 phosphorylation and upregulating Trx1 mRNA and protein expression. (a) Phospho-ASK1 and ASK1 expressions by western blotting ( each). (b) Relative densitometry analysis of the ratio of phospho-ASK1 to ASK1. (c) Trx1 expression by western blotting ( each). (d) Relative densitometry analysis of the ratio of Trx1 to beta-actin. (e) QPCR analysis for Trx1 mRNA in renal cortex. Data are shown as means ± SD ( each). versus CON, versus CON, versus CIN, and versus CIN+NAC.

(a)

(b)

(c)

(d)

(e)