Induction of Expression of p75 Neurotrophin Receptor Intracellular Domain Does Not Induce Expression or Enhance Activity of Mitochondrial Complex II

Figure 1

Subcellular localization of p75NTR and p75ICD in transfected NIH 3T3 and SH-EP1 cells. (a) Immunohistochemical staining with antibodies that stain p75ICD (blue) and its V5 tag (red) demonstrates that p75ICD is present in the nuclei of the cells. Fluorescence microscopy, 100x magnification. (b) Demonstration by Western blot of expression of p75ICD in transfected NIH 3T3 cells. (c) Demonstration by Western blot that transfection of SH-EP1 human neuroblastoma cells with an expression construct for p75NTR results in p75NTR in the nucleus and (predominantly) cytoplasm and p75ICD only in the nucleus.

(a)

(b)

(c)