Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

Figure 4

Effect of A. sinensis peptides on ROS levels of C. elegans. (a) Representative bright-field and corresponding fluorescent images of nematodes captured by ImageXpress Micro System with 20% transmitted light and FITC filter cubes, respectively. The nematodes were pretreated with A. sinensis peptides (AsiPeps) for 24 h prior to exposure to 2 mM paraquat. Scale bars: 200 μm. (b) Effect of A. sinensis peptides (AsiPeps) on ROS level in nematodes exposed to 2 mM paraquat. (c) Effect of A. sinensis peptides (AsiPeps) on ROS level in nematodes undergoing senescence (Day 10). The DCF fluorescence intensity in (b) and (c) was detected by a microplate reader at 485 nm excitation and 520 nm emission, and the data are shown as mean ± SD of three independent experiments. ; .

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