High Fat Diet-Induced Skeletal Muscle Wasting Is Decreased by Mesenchymal Stem Cells Administration: Implications on Oxidative Stress, Ubiquitin Proteasome Pathway Activation, and Myonuclear Apoptosis

Figure 3

Mesenchymal stem cells (MSCs) administration prevents the decreased fiber diameter of the tibialis anterior (TA) muscle in high fat diet- (HFD-) induced skeletal muscle atrophy. TA muscles were obtained from C57BL/10J male mice fed with standard chow (control), a HFD, or a HFD-MSC treated. Muscle cross sections were stained with wheat germ agglutinin to delimit muscle fiber sarcolemma. (a) Images were obtained at 10x and 40x magnification. Bars correspond to 150 μm. (b) Minimal Feret diameters were determined in TA cross sections from (b). Fiber diameters were grouped and ranged from 0 to 70 μm. Values are expressed as a percentage of the total quantified fibers. Values correspond to the mean ± SD (; versus control, two-way ANOVA).

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