High Fat Diet-Induced Skeletal Muscle Wasting Is Decreased by Mesenchymal Stem Cells Administration: Implications on Oxidative Stress, Ubiquitin Proteasome Pathway Activation, and Myonuclear Apoptosis

Figure 4

Systemic mesenchymal stem cells (MSCs) administration prevents the decreased myosin heavy chain (MHC) levels in the tibialis anterior (TA) muscle of mice fed with a high fat diet (HFD). C57BL/10J male mice were fed with a standard chow (control) or HFD for 38 weeks. At week 30, a subgroup of HFD mice received MSC injected through the tail-vein. After eight weeks, all mice were sacrificed and the TA was excised and homogenized to evaluate the following: (a) MHC protein levels through Western blot analysis (GAPDH levels were used as the loading control; molecular weight markers are shown in kDa) and (b) quantitative analysis of the experiments from (a). The levels of MHC normalized to GAPDH are expressed relative to control mice ( versus control; versus HFD).

(a)

(b)