The Peroxidation of Leukocytes Index Ratio Reveals the Prooxidant Effect of Green Tea Extract

Figure 1

Typical overlay dot plots of ratio (ratio of oxidation of the probe C11-BODIPY: FL1/FL2) versus side scatter (SSC): before (T0) and 3 hours (T3) in leukocytes collected after a single dose of two capsules of a green tea extract (GTE) supplement (200 mg × 2). L: lymphocytes, M: monocytes, and G: granulocytes. Unstimulated samples (black) and leukocytes treated with 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH, 10 mM, red), AAPH (10 mM) + 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 10 μM, yellow), phorbol 12-myristate 13-acetate (PMA, 1 μg/mL, blue), or PMA (1 μg/mL) + Trolox (green).