Oxidative Medicine and Cellular Longevity

Research Article

Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

Table 1

¹H NMR and ¹³C NMR spectral data for compounds 1–4 ( in ppm, in Hz).


	1		2		3		4
	δ_H	δ_C	δ_H	δ_C	δ_H	δ_C	δ_H	δ_C

	7.50 (1H, d, 15.8)	141.31	4.70, d, (1H, 7.5)	78.65	4.72, d, (1H, 7.5)	78.85	—	—
	8.24 (1H, d, 15.8)	130.60	2.99, dd, (1H, 16.7, 3.0)	41.00	3.02, dd, (1H, 16.7, 3.0)	41.00	—	—
	—	—	2.81, dd, (1H, 16.7, 13.2)	—	2.82, dd, (1H, 16.7, 13.2)	—	—	—
C=O	—	202.69	—	204.20	—	204.20	—	—
1	—	142.55	—	128.49	—	128.82	—	—
2	7.12 (2H, m, H-2, 6)	128.43	7.22 (1H, dt, 8.5, 2.0)	131.38	7.26 (1H, d, 2.0)	114.81	—	198.21
3		126.57	6.71 (1H, dt, 8.5, 2.0)	116.27	—	144.61	7.97, d (1H, 10.1)	143.48
4	7.46 (3H, m, H-3, 4, 5)	126.46	—	164.03	—	145.05	7.70, d (1H, 10.1)	126.71
4a	—	—	—	—	—	—	—	105.97
5		128.57	6.51 (1H, dd, 8.5, 1.9)	114.86	7.0 (1H, d, 8.0)	114.59	—	143.48
6		130.60	7.26 (1H, dd, 8.5, 2.0)	130.16	7.67 (1H, dd, 8.0, 2.0)	146.64	6.47, s	106.82
7							—	163.17
8								105.07
8a								159.82
1′	—	103.79	—	120.52	—	104.76	—	204.21
2′	6.92 (1H, s, OH-2′)	157.57	—	164.03	—	164.13	7.32, d (1H, 15.4)	142.53
3′	6.52 (1H, d, = 2.4)	114.82	6.68, s	111.30	—	107.88	7.47, d (1H, 15.4)	132.26
4′	6.81 (1H, s, OH-4′)	148.36	—	144.59	—	165.34	—	132.68
5′	6.42 (1H, dd, 8.6, 2.4)	107.52	6.44 (1H, d, 8.9)	108.67	6.68, s	162.45	6.27, 1H, m	107.14
6′	—	161.21	7.71 (1H, d, 8.9)	131.78	—	164.04	—	163.56
7′							6.32 (1H, dd, 1.7, 1.7)	106.29
8′							—	166.12
9′							6.40, 1H, m	101.23
1′′	Glc-6: 5.02 (1H, d, 7.4)	106.19	Glc-2: 5.02 (1H, d, 7.3)	106.12	Glc-3: 4.85 (1H, d, 7.5)	106.07	Rham-1: 5.05, brs	100.8
2′′	3.51 (1H, m, H-2′′, 3′′)	78.36	3.53 (1H, m, H-2′′, 3′′)	78.84	3.79 (1H, t, 11.0)	74.73	3.89, m	71.50
3′′		75.59		74.02	3.55 (1H, m)	78.85	3.12, m	71.62
4′′	3.43 (1H, m, H-4′′, 5′′)	71.82	3.44 (1H, m, H-4′′, 5′′)	71.81	3.5 (1H, m)	70.27	3.12, m	72.76
5′′		71.17		71.23	3.43 (1H, m)	77.32	3.89, m	68.32
6′′	3.88 (2H, dd, 2.0, 12 H-6′′)	66.11	3.78 (2H, dd, 2.0, 12 H-6′′)	61.33	4.30 (2H, dd, 2.0, 12 H-6′′)	61.33	1.15 (3H, d, 7.0)	17.98
1′′′					Glc-6: 5.01 (1H, d, 7.3)	104.75
2′′′					3.52 (1H, m, H-2′′′, 3′′′)	78.65
3′′′						74.03
4′′′					3.44 (1H, m, H-4′′′, 5′′′)	71.25
5′′′						78.01
6′′′					3.88 (2H, dd, 2.0, 12 H-6′′′)	62.16
OMe					3.85, s	56.78	3.39, s, 3.46, s	60.69, 55.79
OH-4	9.10, s
OH-2′	13.2, s
OH-4′	6.81, s
OH-7′							13.59, s
OH-7							14.15, s