Preexposure to Olive Oil Polyphenols Extract Increases Oxidative Load and Improves Liver Mass Restoration after Hepatectomy in Mice via Stress-Sensitive Genes

Figure 3

Real-time quantitative PCR analysis of (a) GCSc gene, (b) HO-1 gene, and (c) Nrf2 gene expression levels in a regenerating liver at different time intervals after hepatectomy. Total RNA, isolated from the liver of three animals, was pooled, subjected to a reverse transcription, and the resulting first-strand cDNA was amplified using primers specific for the mouse GCSc, HO-1, and Nrf2 gene. The results are expressed as the relative ratio of each cDNA level over vehicle control (PSS or PFE) after normalization to corresponding 18S RNA levels. Data represent mean ± SD of five to six animals per group (), and each determination was conducted in duplicate; (A) significantly different from the PSS vehicle control mice at a given time point; (B) significantly different from the PFE vehicle control mice at a given time point.

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