Research Article

Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes

Figure 2

High cholesterol diet induces both Cyp2E1 overexpression and activity. Whole cell lysate was obtained from CW and HC cells treated or not with ethanol (Et, 100 mM) or acetaldehyde (Ac, 200 μM) and subjected to Western blotting. (a) Representative image of the Cyp2E1 immunoblot. (b) Densitometric analysis of protein content relative to actin used as loading control. (c) Cyp2E1 activity. (d) Protein oxidation determined by Oxyblot kit. Each column represents mean ± SEM of at least four independent experiments. Differences were considered significant at versus NT cells. Images are representative of at least four independent experiments.