Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes
Cholesterol induces endoplasmic reticulum stress. Whole cell lysate was obtained from CW and HC cells treated or not with ethanol (Et, 100 mM) or acetaldehyde (Ac, 200 μM) and subjected to Western blotting. (a) Representative image of PERK immunoblot. (b) Densitometric analysis of protein content relative to actin used as loading control. (c–h) ATF6 nuclear translocation determined by confocal microscopy. ATF6 is in red and nucleus in blue. Images are representative of at least four independent experiments. Original magnification 320x.