Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes

Figure 2

Cell viability after treatments. Enterocytic cells after isolation from the rat intestine and exposed to their different treatments. (a) Control cell after 1 hour in DMEM plus antibiotics. (b) Cells exposed for 30 min to DMEM medium with 0.5 mM of H₂O₂ first and then transferred to base DMEM medium, for 30 min each. (c) Cells exposed to DMEM-0.1 mM gallic acid first for 30 min and afterwards transferred to H₂O₂ for 30 min for each treatment. Measurements of viable cells were made by using 0.1% trypan blue exclusion assay with a Neubauer chamber average of percentage of viability reported in Results. Cell viability and SD noted for each treatment. Scale bar 40 μm.

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