Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes

Figure 5

Results obtained with FTIRM infrared spectra data using PCA. (a) Loading plot from the FTIRM samples observed in both protein region (1800–900 cm⁻¹) and lipid region (3200–2800 cm⁻¹) for PC-1 and PC-2. (b) Score plot from the FTIRM samples in the protein wavenumbers (1900–900 cm⁻¹) and the lipid zone of wavenumbers (3200–2800 cm⁻¹) for PC-1 and PC-2. The number of samples used per treatment was 30. Main changes are observed as a shift in amide I (1660 cm⁻¹) and changes in the relations between CH₂ (2920 cm⁻¹) and CH₃ (2960 cm⁻¹) bonds.

(a)

(b)