A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma

Table 1

Resolution of the analytes studied.

Analyte	Rt (min)	Wavelength (nm)	Width (min)	Resolution
Retinol	8.54	330	0.6	N.d.
Astaxanthin	34.07	450	0.38	2.3
Lutein	36.55	450	0.72	1.4
Zeaxanthin	38.24	450	0.53	2.2
E--apo-8′-carotenal	40.01	450	0.29	7.7
Cryptoxanthin	43.94	450	0.22	6.9
15-Z--carotene	46.96	450	0.22	1.4
13-Z--carotene	47.57	450	0.21	1.3
-carotene	48.12	450	0.2	2.9
-carotene	49.57	450	0.31	2.3
9-Z--carotene	50.76	450	0.2	33.0
E-lycopene	64.29	450	0.21	33.0

N.d.: not determined.
= 2[(Rt)  − (Rt)]/( + ).
= resolution.
Rt = retention time.
= width.