Research Article
In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet
Figure 1
Liver of rats submitted to control diet for 2 weeks. (a) Representative photomicrographs of PAS reaction for glycogen, abundant in all hepatocytes. (b) Nile Red-induced fluorescence of small neutral lipid droplets in the perisinusoidal cytoplasm of hepatocytes. (c) Diaminobenzidine-Mn2+-Co2+ reaction for Reactive Oxygen Species (ROS); intense reaction in periportal hepatocytes and occasional Kupffer cells in the midzone or pericentral regions. (d) Immunoreaction against dinitrophenyl (DNP) groups for demonstrating carbonyl groups derivatized with dinitrophenyl hydrazine (DNPH); these are present in perisinusoidal and canalicular membrane domains of hepatocytes. (e) Immunoreaction for visualizing the expression of Mn-dependent Superoxide Dismutase 2 (SOD2); moderately intense staining in the cytoplasm of periportal and pericentral hepatocytes. P: branch of portal vein; CL: branch of centrolobular vein. Scale bar: 50 μm (insets in (d) and (e) represent image details at higher magnification).
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