Subnormothermic Perfusion in the Isolated Rat Liver Preserves the Antioxidant Glutathione and Enhances the Function of the Ubiquitin Proteasome System

Figure 2

Lipid and protein oxidation in isolated perfused rat livers. IPR livers were perfused in a nonrecirculating system with Krebs-Henseleit buffer at 37°C for 15 min, for stabilisation, and then at the indicated temperatures for 15 min more. Lipid peroxidation was measured as TBARS (a) and by western blotting of HNE-protein adducts (b), and advanced oxidation protein products (dityrosine-containing and cross-linking protein products) measured spectrophotometrically at 340 nm (c). Values expressed as mean ± SEM of . Significant differences from livers perfused at 37°C: . Significant differences from livers perfused at 26°C: .

(a)

(b)

(c)