Xanthine Oxidase-Derived ROS Display a Biphasic Effect on Endothelial Cells Adhesion and FAK Phosphorylation

Figure 2

Hx-XO-derived ROS induced time-dependent biphasic FAK tyrosine phosphorylation. HUVECs were treated with Hx-XO (4.5 mU/mL) for the indicated times after which cells were harvested and the soluble fraction analysed for FAK activation. (a) FAK was immunoprecipitated using a monoclonal antibody, and its phosphorylation state and expression were analysed by Western blot (WB) with anti-phosphotyrosine and anti-FAK antibodies. (b) Phosphorylated FAK from different experiments was quantified by densitometry and corrected for the amount of FAK which was quantified by densitometry. Results are expressed as mean ± SEM, . versus untreated cells.

(a)

(b)